High-Throughput Transcriptomics (HTTr)

With the advent of next generation sequencing, the cost for sequencing has drastically reduced over the past decades, yet the practicality to run large projects remains limited. This makes high-throughput research and pre-clinical studies with transcriptomic endpoints costly and often unfeasible.

Maximise sequencing throughput

BioClavis circumvents these issues by leveraging the highly efficient TempO-Seq® transcriptomic/genomic platform developed by BioSpyder. TempO-Seq is specifically designed for very efficient profiling of molecular signatures or whole transcriptomes and is therefore ideal for high-throughput transcriptomic studies. Using a targeted approach with next generation sequencing readout, TempO-Seq maximises the sequencing throughput and allows for indexed pooling of up to 6144 samples per sequencing run.

We can work with you to incorporate high-throughput transcriptomics in your in vitro or in vivo system.

Targeted but not limited

TempO-Seq is a highly flexible assay, both in terms of gene multiplexing (custom panels ranging from tens to thousands of genes up to whole transcriptome) and input (crude lysates, FFPE tissue or purified RNA/DNA). Currently, we offer S1500(+) surrogate panels, specifically designed for economical transcriptome-wide or pathway based HTTr, for human, rat, mouse, and zebrafish. Alternatively, we also offer whole transcriptome panels for human, rat, and mouse. Custom panels can be designed for any species of interest, for any number of genes, or variants and isoforms.

Simplify bioinformatics and pathway analysis

In the TempO-Seq assay, the probes do most of the bioinformatics work for you: since the input of sequencing is clearly defined, read alignment time is significantly reduced. The data will be supplied to you as a .csv file containing gene name and read counts per sample.

Importantly, the data are delivered in a standard format that can be used for essentially any secondary analysis pipeline, for example using commercial or open-source pathway analysis packages such as BMDExpress or Connectivity Map (CMap), to perform Mode of Action (MOA) analysis or identify on/off-target impacts of treatment.

We also offer secondary analysis as a service tailored to your project upon request. 

To find out more, please contact us.